RNase I, 1,000 URemove unwanted RNA by digesting with this heat labile, non sequence specific ribonuclease Complete: Unlike RNase A, RNase I does not have a base preference and digests between all dinucleotide pairs in single stranded RNA Heat Labile: RNase I is easily inactivated by heating 70C for 15 minutes RNase I degrades single stranded RNA to nucleoside 3 monophosphates via 2,3 cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3